Reporter

Part:BBa_K1922010

Designed by: WANG HONGNING   Group: iGEM16_Tongji_Shanghai   (2016-10-14)


luciferase

After inserting the HSP70-p53 fraction into pGL3 vector which contains a luciferase gene, we could obeserve if the tumor cells had been heat treated at in vivo bioluminescence imaging machine with the injection of luciferase substance.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 543
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 864
    Illegal BamHI site found at 696
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 898
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None