Coding

Part:BBa_K1916000

Designed by: Jonathan Chen   Group: iGEM16_UC_Davis   (2016-10-09)


NpF2164g5 + intein-CBD tag

A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and appears vibrant blue-green under normal light.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 531
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 531
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 531
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 531
    Illegal NgoMIV site found at 1027
    Illegal AgeI site found at 1117
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None