Measurement

Part:BBa_K1913035

Designed by: Tianhe Wang   Group: iGEM16_Wageningen_UR   (2016-10-14)

Constitutive promoter with Guanine riboswitch BS-yxjA with mRFP

Measurement device includes a constitutive promoter, guanine riboswitche BS-yxjA and mRFP. The binding of these metabolites (guanine molecule) to the riboswitches on the 5’ UTR of the mRFP mRNA will attenuate translation of mRFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 814
    Illegal AgeI site found at 926
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None