Composite

Part:BBa_K1913029

Designed by: Tianhe Wang   Group: iGEM16_Wageningen_UR   (2016-10-14)


Synthetic plac-FixK2 hybrid promoter +RBS with mRFP

Synthetic plac-FixK2 hybrid promoter composite part which includes mRFP reporter (BBa_E1010). After co-transformation with light sensor BBa_K1913033, mRFP fluorescence could be detected after 12 hours of growth. Please check https://parts.igem.org/wiki/index.php?title=Part:BBa_K1913022 for characterization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 95
    Illegal NheI site found at 118
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 48
    Illegal AgeI site found at 717
    Illegal AgeI site found at 829
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None