Reporter

Part:BBa_K1913020

Designed by: Tianhe Wang   Group: iGEM16_Wageningen_UR   (2016-10-07)


mRFP with degradation tag

mRFP reporter with RBS(BBa_K1491009) tagged with wild type ssrA degradation tag (BBa_M0050), which makes the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease as well as ClpA protease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None