Regulatory

Part:BBa_K1913008

Designed by: Carina Nieuwenweg   Group: iGEM16_Wageningen_UR   (2016-10-13)


Vitamin B12 riboswitch

Constitutive promoter (part J23100), vitamin B12 riboswitch and ssRA Tag. This part allows for regulation of gene expression based on vitamin B12 concentration.

Usage and Biology

This part was used in BBa_K1913011 with a RFP reporter. Please check: http://parts.igem.org/Part:BBa_K1913011# for characterization.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 199
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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