Composite

Part:BBa_K1911007

Designed by: Jackson Harris   Group: iGEM16_LambertGA   (2016-10-21)


pLamdaR-LacI-GFP-pLac-ClpXP-CI

This construct is an example of the switch system that was used in our project. Once the E. coli cells containing our construct was induced with IPTG, the pLamdaR pathway would be shut down while the pLac pathway would be turned on. This allows for the degradation of the gene of interest in which this case is the eGFP. This construct is the finished product of our project after using 3A Assembly to assemble the two different promoter pathways. This construct is switchable because IPTG can be added to switch the system from one pathway to another. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1257
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1221
    Illegal BglII site found at 3074
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2556
    Illegal AgeI site found at 3294
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1900


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