Part:BBa_K1911004

pLamdaR-LacI-GFP-LAA-pLac-ClpXP-CI

This construct is an example of the switch system that was used in our project. Once the E. coli cells containing our construct was induced with IPTG, the pLamdaR pathway would be shut down while the pLac pathway would be turned on. This allows for the degradation of the gene of interest in which this case is the eGFP. This construct is the finished product of our project after using 3A Assembly to assemble the two different promoter pathways. This construct is switchable because IPTG can be added to switch the system from one pathway to another. NOTE: There is no Biobrick scar between Bba_K1911005 and Bba_M0050. The Sequence given will contain a scar of, "tactagag" which is to be ignored.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1257
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1221
  Illegal BglII site found at 3115
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2597
  Illegal AgeI site found at 3335
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1900