Part:BBa_K191008

Promoter LacI - RBS - LovTAP(ILE427PHE) - Term

Mutated version of BBa_K191003 : the amino acid #427 ILE was mutated into PHE.

Usage and Biology

The reasons and choix of this mutation are given by the results of modeling, under [http://2009.igem.org/wiki/index.php?title=Team:EPF-Lausanne/Results/Mutations Mutations]' section.

The mutated version of LovTAP should react faster and the conformational change should be more stable ( the protein stays in the active form for longer).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 370
  Illegal PstI site found at 627
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 370
  Illegal PstI site found at 627
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 370
  Illegal PstI site found at 627
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 370
  Illegal PstI site found at 627
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 764