Part:BBa_K1909009

Weak Anderson / mTaz

BBa_K1909009 is a construct of medium [0.33] Anderson promoter BBa_K608007, a weak [0.6] ribosome binding site and the receptor mTaz, part BBa_K1909002, capable of detecting meso-2,6-Diaminopimelic acid (mDAP).

Usage and Biology

The receptor mTaz is based on the chemotaxis receptor Tar, part BBa_C0082, capable of sensing aspartate and is in combination with the cytosolic domain of osmosensor kinase EnvZ. The combination of BBa_K608007 and BBa_C0082 is part BBa_K1909012.

Functional Parameters

This combination has the lowest expression of the three combinations BBa_K1909007, BBa_K1909008 and BBa_K1909009. Testing the combination showed that the cells grew very, very slow (for miniprep culture more than 48 hours, for colonies on LB-agar plates more than 72 hours).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 174