Signalling

Part:BBa_K1909001

Designed by: Daniel Cortés   Group: iGEM16_Hamburg   (2016-10-13)


pathogen associated molecular pattern sensor

We created a fusion protein, joining the EnvZ two-component system signalling domain to the sensing leucine-rich repeat domain of the pattern recognition receptor NOD1. The EnvZ kinase domain autophosphorylates after target binding and activates OmpR, a prokaryotic selective transcription factor. Therefore it is able to sense pathogen associated molecular patterns (e.g. LPS, mDAP) and subsequently activate reporter expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 171
    Illegal SapI.rc site found at 523


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Categories
Parameters
None