Composite
HBsAg

Part:BBa_K1908002

Designed by: Brian Yang, Jonathan Krog, Sunil Deochand, Jonathan Kelly   Group: iGEM16_Stony_Brook   (2016-10-14)


Blue Chromoprotein + 2a + HBsAg + Yeast Secretion Tag + ADH1 Terminator

The Hepatitis B Surface antigen (HBsAg) is a subunit from the surface antigen of the Hepatitis B virus. The blue chromoprotein is added as a visual indicator of the expression of the antigen. The 2A is a short amino acid sequence that is commonly used by picornaviruses to cleave proteins, and will allow the separation of the blue chromaprotein from HBsAg. The yeast secretion tag will allow the antigen to be secreted into the media. The supernatant of the media can be tested for the secretion of the antigen using an HBsAg ELISA Kit. This composite part also contains a yeast ADH1 Terminator. We hope that this part would serve as a dual reporter in more complex genetically engineered systems.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1091
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

output-NA-
protein-NA-
[edit]
Categories
//chassis/eukaryote/yeast
//chassis/prokaryote/ecoli
//classic/composite/uncategorized
Parameters
output
protein