Coding
Cripto-1

Part:BBa_K1908001

Designed by: Ryan Kawalerski, Tasnia Islam, Joshua Hombrebueno, Sarah Heacox, Samara Khan, Daniel Thach, Meghan Bialt deCelie.   Group: iGEM16_Stony_Brook   (2016-10-14)


Coding sequence for Homo sapien Cripto-1 membrane protein.

This part is a semi-composite part that was synthesized by IDT which holds a Saccharomyces cerevisiae Kozak sequence, a coding sequence for the Homo sapien membrane protein Cripto-1, and an ADH1 Terminator region. The coding sequence was codon-optimized for expression in yeast using the proprietary codon optimization program on the Integrated DNA Technologies website. Our design was intended to induce a strong expression of Cripto-1 in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 172


Functional Parameters

protein-NA-
signalling_molecule-NA-
[edit]
Categories
//cds/membrane/receptor
//chassis/eukaryote/yeast
Parameters
protein
signalling_molecule