Composite
BY-Toggle

Part:BBa_K1908000

Designed by: Ryan Kawalerski, Sarah Heacox, Jonathan Krog   Group: iGEM16_Stony_Brook   (2016-10-14)


Blue-Yellow fluorescent protein toggle switch using pLac and OmpC promoter.


This construct is a blue-yellow color toggle that expresses fwYellow (K1033910) and amilCP (K592009) dependent on the activation of the OmpC promoter at varied ambient osmolarities. A negative feedback loop inhibits production of fwYellow (regulated by pLac) when the OmpC promoter is active and amilCP/LacI is being produced. Growth in high-Osm media is expected to give a blue color while growth in low-Osm media is expected to produce a yellow color.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2896
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 190
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 567

Functional Parameters

color-NA-
emission-NA-
[edit]
Categories
//function/reporter/color
//function/sensor
Parameters
color
emission