Signalling

Part:BBa_K1904008

Designed by: Roger Milton Rubio S�nchez, Enrique Chimal, Enrique Palomino, Brian Morteo   Group: iGEM16_TecCEM   (2016-06-07)


TAT signal peptide with inducible promoter and RBS

IPTG inducible promoter and RBS (BBa_J04500) was assemble with BBa_K1904007, resulting on the TAT signal peptide for protein export in Outer Membrane Vesicles (OMVs). This biobrick is reported in RFC25 to allow protein engineering avoiding stop codons in between the protein of interest and the peptide.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None