Intermediate

Part:BBa_K1904004

Designed by: Enrique Chimal Juarez, Roger Milton Rubio S�nchez, Enrique Palomino, Brian Morteo   Group: iGEM16_TecCEM   (2016-06-07)

T4 Holin with RBS (RFC 25 N-Part).

This biobrick contains the coding sequence for a T4 Holin (BBa_K1904003) which promotes pore formation on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part, designed in RFC25, is assembled with B0034 (RBS). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the biobrick is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None