Device

Part:BBa_K190039

Designed by: Nienke Kuipers   Group: iGEM09_Groningen   (2009-09-08)

Arsenic Accumulation device (Low constitutive promoter + fMT + GlpF)

A device which can be used to accumulate As(III) without (under certain concentrations) toxifying E. coli. The expression of both genes is constitutively expressed (under low constitutive promoter).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 81
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 170
    Illegal NgoMIV site found at 554
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None