Composite

Part:BBa_K190035

Designed by: Michael Verhoeven   Group: iGEM09_Groningen   (2009-09-07)


GVP with CueO regulated promoter

The Gas Vesicle gene cluster (BBa_I750016) submitted by Melbourne 2007 team and the metal sensitive promoter BBa_K190017 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 385
    Illegal BamHI site found at 4190
    Illegal XhoI site found at 3878
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 1238
    Illegal AgeI site found at 4157
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2648
    Illegal SapI.rc site found at 3804


[edit]
Categories
Parameters
n/aGVP with CueO regulated promoter