Composite
Part:BBa_K190033
Designed by: Michael Verhoeven Group: iGEM09_Groningen (2009-09-07)
GVP with ArsR regulated promoter
The Gas Vesicle gene cluster BBa_I750016 submitted by Melbourne 2007 team and the metal sensitive promoter BBa_K190015 were combined, and the product ligated into vector pSB1AC3. The construct was used to test and caracterize the gas vesicle formation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 414
Illegal BamHI site found at 4219
Illegal XhoI site found at 3907 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 865
Illegal NgoMIV site found at 1267
Illegal AgeI site found at 4186 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2677
Illegal SapI.rc site found at 3833
[edit]
Categories
Parameters
n/a | GVP with ArsR regulated promoter |