Coding

Part:BBa_K1900003

Designed by: Elise Sloey   Group: iGEM16_WLC-Milwaukee   (2016-10-08)


E. coli tolC

This is the E. coli K-12 tolC gene which produces the E. coli TolC protein. This protein is necessary for antibiotic efflux and was used as a control for our project. TolC proteins can be used to screen for bacteriophages, this is the central idea of our project.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 265
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 874
    Illegal NgoMIV site found at 1144
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1110


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