Regulatory

Part:BBa_K1899000

Designed by: iGEM16_HKUST   Group: iGEM16_Hong_Kong_HKUST   (2016-10-18)


tetp-BFP-phlFp-RFP-lacp-GFP

It is the main construct which is expected to perform as a tristable switch with three discrete, but alternating, steady states. By applying different inducers, three repressible promoters would act accordingly, producing three different outputs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1858
    Illegal BglII site found at 3891
    Illegal XhoI site found at 7477
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 7778
    Illegal AgeI site found at 5191
    Illegal AgeI site found at 5303
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None