Regulatory

Part:BBa_K1897040

Designed by: Nguyen Hoang Diem Phuong   Group: iGEM16_NUS_Singapore   (2016-10-14)


Modified lldPRD operon promoter

This is the shorter version of the promoter region for the lldPRD operon (previously named lct) of E. coli (Part:BBa_K822000) which begins from operator site O1. The lldPRD operon encodes a lactate-dehydrogenase, and the lldPRD promoter has been shown to be induced by L-lactate.

The promoter is repressed/activated by the regulatory protein lldR encoded by the operon. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None