Regulatory
Part:BBa_K1897040
Designed by: Nguyen Hoang Diem Phuong Group: iGEM16_NUS_Singapore (2016-10-14)
Modified lldPRD operon promoter
This is the shorter version of the promoter region for the lldPRD operon (previously named lct) of E. coli (Part:BBa_K822000) which begins from operator site O1. The lldPRD operon encodes a lactate-dehydrogenase, and the lldPRD promoter has been shown to be induced by L-lactate.
The promoter is repressed/activated by the regulatory protein lldR encoded by the operon. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |