Part:BBa_K1897005

HasI sigma factor coding sequence

Usage and Biology

HasI ia a 19 kDa protein that acts as an extra cytoplasmic function sigma factor under the Has operon system. The Has system is a heme acquisition system originally from the Serratia marcescens. HasI is a componentof the system and this cytoplasmic protein is usually bound to the anti-sigma factor HasS and therefore is inactive. When the holo-HasA hemophore binds to the cell surface receptor HasR, it triggers a conformational change in HasS. This then releases the HasI which will then trigger expression of genes under the control of the Has promoter.

Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 42
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]