Generator

Part:BBa_K1896014

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-13)


mGFPuv2-streptavidin generator

This part constitutively expresses a fusion protein comprising the monomeric GFP variant mGFPuv2 and Streptavidin. When expressed in E. coli, less fluorescence is observed compared to BBa_K1896010. Growth is partially inhibited as well, probably due to the tetrameric nature of the Streptavidin domain, which may cause aggregation of the fusion protein. BBa_K1896015 contains a monomeric variant of Streptavidin and performs better.

MGFPuv2 can be used in difficult fusion proteins, from left to right: non-fluorescent control, INPRC-mGFPuv2 (weak promoter), mGFPuv2-streptavidin, mGFPuv2-mSA2, mGFPuv2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 872
    Illegal AgeI site found at 923
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None