Coding

Part:BBa_K1896008

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-13)


INP_RC

INPRC is a truncated variant of the inaZ from Pseudomonas syringae (Part:BBa_K1896001). The N-terminal domain was removed in order to prevent localisation to the outer membrane [1], while still maintaining ice nucleation activity [2]. No stop codon is present in this part to allow seamless assembly of fusion proteins.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 142
    Illegal NgoMIV site found at 1654
    Illegal NgoMIV site found at 1798
    Illegal NgoMIV site found at 2062
    Illegal NgoMIV site found at 2206
    Illegal NgoMIV site found at 2254
    Illegal NgoMIV site found at 2374
    Illegal AgeI site found at 1318
  • 1000
    COMPATIBLE WITH RFC[1000]

References

  1. Li, L., Gyun Kang, D., & Joon Cha, H. (2004). Functional display of foreign protein on surface of Escherichia coli using N‐terminal domain of ice nucleation protein. Biotechnology and bioengineering, 85(2), 214-221.
  2. Green, R. L., Corotto, L. V., & Warren, G. J. (1988). Deletion mutagenesis of the ice nucleation gene from Pseudomonas syringae S203. Molecular and General Genetics MGG, 215(1), 165-172.


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