Coding

Part:BBa_K1896003

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-12)


INP_NC (opt)

This is an E. coli codon optimised version of BBa_K811003, which encodes a truncated variant of an ice nucleating protein (INP) found in Xanthomonas campestris. The truncated protein INPNC lacks the large central repeat region, but retains membrane display abilities. The stopcodon and one BsaI site were removed from the recoded sequence.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None