Composite

Part:BBa_K1893020

Designed by: Akash Bhattacharjee, Henry Lloyd-Laney   Group: iGEM16_Imperial_College   (2016-10-14)


Las receiver with STAR (LasR+pLas+STAR)

This is the STAR sequence under control of the Las quorum signalling system. The gene encoding the transcriptional activator LasR is downstream a constitutive Anderson promoter, j23101. LasR is activated by 3O-C12-HSL. Included in the construct is the LasR-activated promoter pLas.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None