Composite

Part:BBa_K1893012

Designed by: Akash Bhattacharjee, Gear Rotrattanadumrong   Group: iGEM16_Imperial_College   (2016-10-14)


Constitutive STAR and anti-STAR (J23119+STAR+anti-STAR)

To regulate STAR activity, we designed an inhibitory RNA sequence (Anti-STAR). The Anti-STAR sequence is identical to a portion of the pAD1.S5 attenuator sequence, and is therefore also complementary to STAR. Anti-STAR has a higher affinity for STAR than the pAD1 attenuator does, so, when transcribed, Anti-STAR sequesters STAR and prevents it from interacting with the target sequence and activating transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 154
    Illegal NheI site found at 177
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None