Part:BBa_K1893011
Constitutive anti-STAR (J23119+anti-STAR)
This is the Anti-STAR sequence (BBa_K1893014) under control of the constitutive Anderson promoter j23119. Anti-STAR is identical to part of the STAR-target attenuator sequence (pAD1.S5) but much shorter in length. When transcribed, it is fully complementary and thus binds to the STAR-antisense RNA (pAD1.A5) with much higher affinity than the STAR-target sequence.
Usage and Biology
The anti-STAR DNA sequence is identical to part of the 5’ end of the STAR target DNA sequence, but shorter in length. Anti-STAR was designed as an inhibitory RNA sequence for STAR. When transcribed, it produces a RNA product that is fully complementary to the transcribed antisense-STAR product. Anti-STAR has a higher affinity for STAR than the STAR target sequence, which allows it to sequester STAR and prevents it from binding to the STAR target sequence to activate transcription of a downstream coding sequence.
Anti-STAR was designed by using RNAstructure, a software tool that allowed us to predict a complementary RNA sequence to STAR that binds with higher affinity to STAR than it does to the full STAR target sequence.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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