Device
Part:BBa_K189104
Designed by: GuoQiang Chen Group: iGEM09_Tsinghua (2009-10-20)
Therapeutic DNA
Therapeutic DNA containing a cos site, Amp resistance gene and O, P genes for replication.
We have characterized the co-functioning of this part with our designed GenSniper genome. However, whether a stuffer is needed for the package of Therapeutic DNA into GenSniper viroin should be further identified.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 53
Illegal EcoRI site found at 4411
Illegal SpeI site found at 3236
Illegal PstI site found at 1547 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 53
Illegal EcoRI site found at 4411
Illegal NheI site found at 1388
Illegal SpeI site found at 3236
Illegal PstI site found at 1547 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 53
Illegal EcoRI site found at 4411
Illegal BglII site found at 3997
Illegal BglII site found at 4057
Illegal BamHI site found at 5734
Illegal XhoI site found at 3795 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 53
Illegal EcoRI site found at 4411
Illegal SpeI site found at 3236
Illegal PstI site found at 1547 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 53
Illegal EcoRI site found at 4411
Illegal SpeI site found at 3236
Illegal PstI site found at 1547
Illegal AgeI site found at 2941
Illegal AgeI site found at 3053
Illegal AgeI site found at 5695 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 910
Illegal BsaI site found at 1622
[edit]
Categories
Parameters
None |