Part:BBa_K1891014
P(3HB)(BBa_K934001) + HSP promotor (BBa_K873002)
Poly-3-hydroxybutyrate, P(3HB), a kind of PHAs is synthesized by three enzymes.The A gene encodes for the 393 amino acids protein, 3-ketothiolase (PhaA). The B1 gene encodes for the 246 amino acids protein, acetoacetyl-CoA reductase (PhaB). The C1 gene encodes for the 589 amino acids protein, PHA Synthase (PhaC) These three enzymes can use Co-A as a substrate to produce P(3HB). IGEM team Tokyo Tech2012 designed and synthesized phaC1-A-B1gene sequence BBa_K934001, and expressed via constitutive promoter. In order to make the production process more controllable, we added arabinose inducible promoter（BBa_I0500）to phaC1-A-B1 gene sequence (BBa_K934001), constructing the arabinose inducible parts(BBa_K1891014）. Finally we linked the newly synthesized part to the standard vector pSB1C3. The new plasmids can be used directly for arabinose induced expression in E.coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2134
Illegal BglII site found at 2959
Illegal BamHI site found at 1144 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1440
Illegal NgoMIV site found at 1511
Illegal NgoMIV site found at 2111
Illegal NgoMIV site found at 2423
Illegal NgoMIV site found at 2702
Illegal NgoMIV site found at 3354
Illegal NgoMIV site found at 3376
Illegal AgeI site found at 979 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5220
Illegal SapI site found at 961
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