Reporter

Part:BBa_K1891012

Designed by: Zhao Jingyu   Group: iGEM16_BNU-China   (2016-10-10)

YCE+beta-tubulin

It expresses YCE-beta-tubulin fusion protein with a 42bp linker. The YCE links to the N-terminal of beta-tubulin. We connected this two gene fragments using fusion PCR. And then we cloned it into pET30a(+) and add IPTG until its final concentration is 1mmol/L, incubate for 4-6h(37°C, 250rpm) to induce the expression of this fusion protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 542
    Illegal BamHI site found at 1325
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 583
    Illegal AgeI site found at 391
    Illegal AgeI site found at 721
    Illegal AgeI site found at 1489
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None