Device

Part:BBa_K189101

Designed by: GuoQiang Chen   Group: iGEM09_Tsinghua   (2009-10-20)

pET linkage group of GenSniper via Bottom Up approach

pET linkage group of GenSniper, pET-28a+Nu1+A+W+B.

This part should work with BBa_K189102 for production of GenSniper as described in Tsinghua iGEM09 project. We have identified that BBa_K189101 and BBa_K189102 together in BL21 DE3 can produce desired protein complex. But their function with Targeted BioBrick(BBa_K189000) should be further identified.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 4225
    Illegal XbaI site found at 4592
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 192
    Illegal NheI site found at 4488
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 192
    Illegal BglII site found at 4658
    Illegal BamHI site found at 4455
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 4225
    Illegal XbaI site found at 4592
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 4225
    Illegal XbaI site found at 4592
    Illegal NgoMIV site found at 4690
    Illegal NgoMIV site found at 6278
    Illegal NgoMIV site found at 6438
    Illegal NgoMIV site found at 9485
    Illegal AgeI site found at 437
    Illegal AgeI site found at 1370
    Illegal AgeI site found at 2434
    Illegal AgeI site found at 3314
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3011
    Illegal BsaI.rc site found at 4145
    Illegal SapI site found at 7358


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Categories
Parameters
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