Part:BBa_K1886014
AND GATE-input_red light+IPTG
The circuit to receive input signals is composed of AND GATE, plac promoter and light-controlled promoter. After being induced by IPTG, plac promoter activates the expression of T7 RNA polymerase (T7 ptag) which contains two amber mutations. The expression of a special tRNA (supD), which is used to identify amber mutation, is activated by Pλ promoter without the restraint of CI protein. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase, and red light could inhibit the produce of CI protein(for more information, see the part"PompC-NOT Gate"). Therefore, when IPTG and red light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 109
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 112
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