Device

Part:BBa_K1886010

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-07)


AND GATE-input_AHL+green light

The circuit to receive input signals is composed of AND GATE, quorum sensing promoter and light-controlled promoter. After being induced by AHL, pLuxR promoter activates the expression of T7 RNA polymerase (T7 ptag) which contains two amber mutations. After being induced by green light, PcpcG2 promoter activates the expression of a special tRNA (supD), which is used to identify amber mutation. In the wild-type E.coli, amber mutation will inhibit the translation of T7 RNA polymerase. Therefore, when AHL and green light exist together, T7 RNA polymerase will be expressed, and then T7 promoter will be activated to express the downstream gene.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 279
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 282
    Illegal BsaI.rc site found at 525


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Categories
Parameters
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