Device

Part:BBa_K1886005

Designed by: Lejian Jiang   Group: iGEM16_ZJU-China   (2016-10-06)


Constitutive promoter-ho1-pcyA

This circuit is composed of the constitutive promoter, ho1 chromoprotein and pcya chormoprotein. They facilitate Membrane protein ccaS and cph8 to be activated by light of certain wavelength.

The HO1 and PcyA genes, encoding heme oxygenase-1 (HO1) and phycocyanobilin (PCB):ferredoxin (Fd) oxidoreductase (PcyA), respectively, are required for chromophore synthesis in photosynthetic photoreceptors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1607
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

BACKGROUND

Overview

Registered Parts in our wiki is the subunit of the entire light-controlled system, each of which has no function. So I will introduce our light input part of our project after the combination of all the subunits in the page of part K1886006

RESULTS


Gel electrophoretic analysis

Fig.1 Fig.1 Gel electrophoretic analyses of PCR products






[edit]
Categories
//cds/reporter/chromoprotein
//chassis/prokaryote/ecoli
//classic/reporter/constitutive
Parameters
None