Coding

Part:BBa_K1885120

Designed by: Kien Patrick Malarney   Group: iGEM16_ASIJ_Tokyo   (2016-10-29)


PETase from Ideonella Sakainesis

Isolated from Ideonella Sakarinesis, this is the coding sequence for the enzyme PETase, which catalyzes the hydrolysis of polyethylene terephthalate into ethylene glycol and terephthalic acid. It codes for a PET hydrolase.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 864
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 864
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 688
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 864
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 864
    Illegal NgoMIV site found at 139
    Illegal NgoMIV site found at 193
    Illegal NgoMIV site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None