Deleted
Sample Discontinued
Experience: Issues
Not Used
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Composite

Part:BBa_K1885000

Designed by: Tomi McGuire, Lisa Watanuki, Kien Malarney and Lauren Sekiguchi   Group: iGEM16_ASIJ_Tokyo   (2016-10-13)


PETase >PSB1C3

The Biobrick was designed to optimize the production of PETase, a useful enzyme to break down PET plastic. This biobrick was made with, Anderson Promotor: BBa_J23100, PETase (derived from Ideonella sakaiensis, and characterized by Keio University)and Osmotically-inducible protein Y


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1127
    Illegal NheI site found at 1150
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 824
    Illegal AgeI site found at 936
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None