Part:BBa_K1884013

Paromomycin

Paromomycin is an aminoglycoside antibiotic, first isolated from Streptomyces krestomuceticus in the 1950s.[1] It was discovered by Parke Davis now Pfizer and introduced as Humatin in 1960.[2] It is also called monomycin and aminosidine;[3]

Figure 1. Biobrick part of Paromoycin.

Biology

Paromomycin is a protein synthesis inhibitor in nonresistant cells by binding to 16S ribosomal RNA.[4] This broad-spectrum antibiotic soluble in water, is very similar in action to neomycin. Antimicrobial activity of paromomycin against Escherichia coli and Staphylococcus aureus has been shown.[5](Fig 2)

Figure 2. The structure of Paromoycin.

Paro is 819bp in length. Fig.3 shows the DNA sequence of Paro is successfully amplified by PCR from psi-Check2 vector. From this electrophoretogram, we can see the brightness of Paro PCR product is rather high compared with DNA Marker, which indicates that the PCR product of Paro is in a high concerntration.

Figure 3. The electrophoretogram of Paromoycin PCR product.

Usage

In our project, paromomycin is for use in Screening monoclone on LB plate. Our team configurated six different concentrations of Paromomycin solutions in order to figure out the best screening concentration of Paromomycin solution. Due to this experiment, we find out the best screen concentration of paromomycin solution is 9ug/ul in LB plate.（Fig.4）

Figure 4. The conlony in LB medium plate containing Paromoycin.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]