Device

Part:BBa_K188244

Designed by: shau-yi wu   Group: iGEM09_NCTU_Formosa   (2009-10-19)

Promoter (lacI regulated, lambda pL hybrid)+RBS+GFP-LVA+terminator

We use this device to measure the expression of promoter lacI/pL(BBa_R0011) which is regulated by the interaction of lacI gene from device A(BBa_K188151) in our project and lactose that we add.

This device is without the tetR gene that we expect to add.

T5.jpg 21.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 848
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 725


[edit]
Categories
Parameters
n/aPromoter (lacI regulated, lambda pL hybrid)+RBS+GFP-LVA+terminator