Coding

Part:BBa_K1882006

Designed by: Milena Krach   Group: iGEM16_Tuebingen   (2016-10-14)


Fructose Transporter

This part encodes a carbohydrate transporter from lactobacillus Johnsonii ATCC 33200 that imports fructose into the target cell. In this process, fructose is phosphorylated by a N-phosphohistidine residue to yield fructose-6-phosphate and histidine. The protein is part of the ABC transporter superfamily. This part ist RFC 10 compatible.The part's codon usage is optimized for expression in lactobacilli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 523
    Illegal NheI site found at 838
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 9
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1678


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Parameters
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