Device

Part:BBa_K188124

Designed by: shau-yi wu   Group: iGEM09_NCTU_Formosa   (2009-10-19)


RBS+luxR repressor/activator

n complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr BBa_R0062, and repressing transcription from Pl BBa_R0063.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aRBS+luxR repressor/activator