Coding

Part:BBa_K1880002

Designed by: Adam Yaseen   Group: iGEM16_Duke   (2016-10-14)


TAT

Taxadienol acetyl transferase (TAT)

taxa-4(20),11-dien-5α-ol + acetyl-CoA → taxa-4(20),11-dien-5-α-yl acetate + coenzyme A

Km: 4.2 uM for taxa-…-dien-…-ol, 5.5 uM for acetyl-CoA (Walker, et al., 1999).

kcat: 29.59 s-1 (Chau, Walker, Long, & Croteau, 2004)

TAT makes use of the endogenous metabolite acetyl-CoA to add an acetyl group to the backbone of the Taxol ring using the hydroxyl group just added to the ring.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None