Part:BBa_K1880001

DBAT

10-deacetylbaccatin III-10-O-acetyl transferase (DBAT)

10-deacetylbaccatin III + acetyl-CoA → baccatin III + coenzyme A

Km: 57.6 uM for 10-deacetylbaccatin III (Ondari & Walker, 2008)

kcat: 0.58 s-1 (Ondari & Walker, 2008)

DBAT provides the final acetylation to the Taxol backbone. It makes use of well characterized substrates, both 10-deacetylbaccatin III and acetyl-CoA to make for easier characterization. The product is also well characterized. It makes use of the 10’ carbon hydroxyl group to add the acetyl group via the CoA thioester mechanism.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Protein expression level: 21.3% from SDS-page gel results <object data="https://static.igem.org/mediawiki/2018/0/08/T--Duke--GelFinal.pdf" width="100%" height="700"> </object> Panel 3 shows the area for corresponding peak that represent DBAT protein expression level.