Part:BBa_K1880000

badA

Benzoate-CoA ligase (BadA)

ATP + benzoate + CoA <-> AMP + diphosphate + benzoyl-CoA

Km: 1 uM (Egland, Gibson, & Harwood, 1995); 4.4 uM (Thornburg, Wortas-Strom, Nosrati, Geiger, & Walker, 2015)

kcat: 28 s-1.

BadA does not interface directly with the Taxol production pathway directly but is involved in the production of a metabolite used for the addition of the benzoyl group to the Taxol backbone, benzoyl-CoA. Benzoyl-CoA does not exist in the metabolome of E. coli, nor does benzoate. However, benzoate is a low cost metabolite and can be bought and added to fermentation if necessary for the project after the Thornburg optimization.

Characterization Data

Joe insert graphs and figure descriptions here

See http://2016.igem.org/Team:Duke/Results for characterization.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 909
  Illegal NgoMIV site found at 1246
  Illegal AgeI site found at 544
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 187