Coding

Part:BBa_K1879000

Designed by: Madeleine Stone   Group: iGEM16_Virginia   (2016-10-12)


CDS of N-CBZ cleavage enzyme

This part contains just the coding sequence for the N-carbobenzyloxy (CBZ)-cleavage enzyme necessary to enantioselectively remove the CBZ protecting group from amino acids. In our biocontainment system, the enzyme serves to cleave CBZ from leucyl-tRNA charged with CBZ-protected leucine. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 311
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI.rc site found at 1334


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Categories
Parameters
None