Coding

Part:BBa_K187421

Designed by: Julia Pon   Group: iGEM09_Alberta   (2009-10-21)


trpE in pBA

trpE encodes anthranilate synthase component I, in the tryptophan biosynthesis pathway. This part is in the standard plasmid pSB1A3. This gene is present in plasmid pBA (part BBa_K187001), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see RFC 47.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 1579
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1579
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 980
    Illegal BglII site found at 1041
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 1579
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 1579
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None