Composite

Part:BBa_K1871010

Designed by: Broad Run High School iGEM Team   Group: iGEM15_BroadRun-NorthernVA   (2015-09-13)

Coding Sequence AMY1


This is an AMY gene obtained from a Bacillus strain of bacteria. It produces an enzyme that hydrolyzes starch and degrades it into monosaccarides.


Native Alpha Amylase Secretion Sequence

This is the Bacillus amyloliquefaciens alpha amylase signal sequence, which directs the protein to be secreted.

Alpha Amylase

α-Amylase is a protein enzyme that hydrolyses alpha bonds of long alpha-linked polysaccharides, such as glycogen.

ADH1 Terminator

This part is the terminator region from yeast alcohol dehydrogenase gene.


Usage and Biology

This part was proved functional by the 2015 BRHS iGEM Team. Please click the below link to see their results. http://2015.igem.org/Team:BroadRun-NorthernVA/Results


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1436
    Illegal NgoMIV site found at 1451
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None