Part:BBa_K1871001

Alpha amylase with native secretion

This is an alpha amylase promoter derived from Bacillus amyloliquefaciens. It is flanked by Kozak sequence the ADH1 terminator. Expression is controlled by the minimal cyc yeast promoter.

Minimal cyc promoter

This part is the center of the CYC1 promoter, S. cerevisiae. This promoter allows basal transcription, and the transcription signal can be modulated by adding operator sites upstream of this BioBrick. This allows activators or repressors to act on the transcription machinery.

Kozak Sequence

This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.

Native Alpha Amylase Secretion Sequence This is the native Bacillus amyloliquefaciens signal sequence, which directs the protein to be secreted.

Alpha Amylase

Obtained from Bacillus amyloliquefaciens, alpha amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.

ADH1 Terminator

This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 105
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1533
  Illegal NgoMIV site found at 1548
  Illegal AgeI site found at 1851
  
• 1000
  COMPATIBLE WITH RFC[1000]