Part:BBa_K187077

terminator reverse strand, Biobytes assembly method

This part can be annealed to the terminator forward strand A (BBa_K187067) or the terminator forward strand B (BBa_K18790), to produce an A or B overhang respectively. The A overhang can anneal to the A' end on the 3' end of a part produced from pBA. The B overhang can anneal to the B' overhang on the end of a part produced from pAB.

The biobytes assembly method can be used to rapidly assemble DNA segments in a particular order. The 3' end of constructs made with this method can be annealed to a terminator segment, which can be digested and annealed to the anchor to circularize the construct.

For more details see http://2009.igem.org/Team:Alberta/DNAanchor, or RFC #47

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]